Composite
Part:BBa_K4164015:Design
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-09-30)
Inductive expression of RXR-ddRFPB1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1964
Illegal NheI site found at 2232 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 454
Illegal AgeI site found at 1481 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 836
Design Notes
We recombined BBa_K4164997 to plasmid pET-29a+ by homologous recombination.
Source
This sequence is composed of BBa_J64997, BBa_K4164995, BBa_K2556021, BBa_K4164997 and BBa_K923004.